Biolegend phospho flow
WebOct 1, 2024 · Protein phosphorylation is a ubiquitous and key step in signaling pathways. For flow cytometry staining, the right protocol can help accurately capture these phosphorylated targets for clear-cut data. Learn … WebAug 2, 2024 · Intracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry 1. Prepare 70% absolute ethanol. Chill solution by storing …
Biolegend phospho flow
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WebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete ... WebImmunogen. Synthetic peptide corresponding to human STAT4 phosphorylated at tyrosine 693. Formulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
WebPhosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. Web• Examine cell signaling networks utilizing advanced quantitative phospho-flow cytometry to characterize individual cancer cells in order to enable …
WebSynthetic phosphopeptide derived from isoform 2 of human p70 S6 Kinase beta-1 (P23443) around the phosphorylation site of threonine 389 (G-F-TP-Y-V). Database link: P23443 General notes The Life Science industry has been in the grips of a reproducibility crisis for a number of years. WebBrand: BD Phosflow™ Alternative Name: CD3ζ Reactivity: Human (QC Testing) Isotype: Mouse IgG2a, κ Immunogen: Phosphorylated Human CD3ζ Peptide Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_647237 Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
WebJul 16, 2016 · BioLegend UC Irvine About An experienced Research Associate with a Master of Science - MS in Biomedical Engineering, and a substantial record of working in both academia and the biotech industry....
WebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization , Foxp3/Transcription Factor Buffer, and IC … how many people live in guilford ctWebJan 21, 2024 · PE anti-SLP76 Phospho (Ser376) Isotype : Mouse IgG1, κ. Other Names : LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein. Size : 25 tests. Price (USD) : 159 USD. how many people live in guadalajaraWebPhospho-specific flow cytometry, or phospho flow, measures the phosphorylation state of intracellular proteins at the single cell level. Many phosphorylation events can be analyzed simultaneously in each cell, along with cell surface markers, enabling complex biochemical signaling networks to be res … how can traveling impact someoneWebJan 21, 2024 · home > BioLegend > Alexa Fluor® 647 anti-STAT1 Phospho (Tyr701) product summary. Loading... company name : BioLegend. ... flow cytometry. more info … how can travel change a person for the betterWebConjugation of monoclonal antibodies: http://www.drmr.com/abcon/ Phospho Flow Buffer Resource: BD FACS Select – Intracellular Buffer Compatabilty Resource Panel Design Tools: MACS Miltenyi Biotech – Panel Builder Fluorish – Panel Builder Biolegend – Multicolor Panel Selector BD Biosciences – BD FACSELECT Multicolor Panel Designer how can traveling be educationalWebPhospho-mTOR (Ser2448) Antibody (12-9718-42) in Flow TOP: Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 and CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81) for 48 hours (right). how many people live in guatemala cityWebFor staining phosphorylated intracellular targets, we've released a video protocol outlining how to stain cells using our True-Phos™ permeabilization buffer. Need a control for your … Intracellular Flow Cytometry Staining Protocol - BioLegend how many people live in halifax