Csfv e2 phosphatase

Author: kkyu

August undefined, 2024

WebThe CSFV E2 protein has been widely used as a major antigen for antibody detection. It is important to improve the affinity between the E2 protein and CSFV antibodies to improve the performance of the detection method. In this study, a recombinant E2 extracellular protein (amino acids 1–331) with a native homodimer conformation and high ... WebMar 29, 2024 · Classical swine fever virus (CSFV) E2 protein, the major virus structural glycoprotein, is an essential component of the viral envelope. E2 is involved in virus absorption, induction of a protective immune …

Crystal structure of glycoprotein E2 from bovine viral diarrhea …

WebOverall, our data suggests that the CSFV E2 and PPP1CB protein interact in infected cells, and that activation of the PP1 pathway decreases virus replication. WebThe computational-designed peptide in this study has high sensitivity and selectivity and is thus useful for the purification of CSFV E2. The novel method of design provided a broad platform and powerful tool for protein-peptide screening, as well as new insights into CSFV vaccine design. shuttlemead bexley

Prediction of phosphorylation sites in CSFV E2 (A) Amino …

WebApr 22, 2024 · Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control … Webfever virus (CSFV), the causative agent of this disease, is an enveloped, positive-strand RNA virus belonging to the genus Pestivirus in the family Flaviviridae. The envelope of CSFV contains three glycoproteins, Erns, E1, and E2.2) Among these, E2 is the most immuno-genic, and is a virulence determinant.3) Post-transla- WebFeb 4, 2024 · A novel CSFV E2 sequence (E2ZJ) was identified from an epidemic strain of Zhejiang for outstanding secretion in baculovirus and enhanced immunogenicity. E2 … the paris of north america

Expression and purification of classical swine fever virus …

Viruses Free Full-Text SERTA Domain Containing Protein 1

WebMar 29, 2024 · Classical swine fever virus (CSFV) E2 protein, the major virus structural glycoprotein, is an essential component of the viral envelope. E2 is involved in virus absorption, induction of a protective immune response and is critical for virulence in … WebTo further confirm the infection of CSFV, a pair of specific primers were designed for detection of CSFV E2 genes in the collected immune organs (tonsil, thymus, spleen and inguinal lymph node) through reverse transcription (RT)-PCR method. Simultaneously, E2 expression in the collected immune organs was analyzed by IHC method exploiting a ... shuttle mco to cocoa beach hotelWebMar 8, 2024 · Author summary Classical swine fever virus (CSFV) is a highly pathogenic RNA virus belonging to Flaviviridae family that can cause deadly classical swine fever (CSF) among pigs. In this study, we identified ADAM17 as a binding partner for CSFV envelope protein E2 using biochemical approaches. Knockout of ADAM17 rendered permissive … the paris new york apartment

"WebFeb 24, 2024 · Classical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and Erns are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and Erns were expressed … " - Csfv e2 phosphatase

Csfv e2 phosphatase

Method for in Vivo Production of Deglycosylated Recombinant …

WebMar 12, 2024 · CSFV E2, CHO-pTxnip-4-rE2 cell clone (F12), was des-ignated as the target cell clone for recombinant E2 protein production in suspension cultured mode. CHO-pCMV-rE2 (A11) and parental CHO cells were conducted the same process as control. In 6 days batch-wise suspension culture performed in 1 L shaking flask with 550 mL SFM, recom-

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WebJan 8, 2014 · E2 is one of the envelope glycoproteins of pestiviruses, including classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV). E2 is involved in several critical functions, including virus entry … WebMar 1, 2008 · The microtiter plate was coated with total soluble proteins from the wild-type and transformed plants and the known CSFV E2 antigen at 4 °C overnight. The wells …

WebMar 29, 2024 · DOE PAGES ® Journal Article: Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta … WebMar 15, 2024 · A series of truncated and overlapping fragments of CSFV E2, shown in Fig. 3, were designed in this study, and the related primers were listed in Table 1.These …

WebDec 5, 2005 · CSFV E1 (B) was detected with an E1 specific rabbit antiserum, and CSFV E2 (C) was detected with mAb WH303. ... slides were incubated with alkaline-phosphatase-conjugated, goat anti-mouse antibody for 20 min at room temperature. Acknowledgments. We thank Aniko Zsak, Adriene Lakowitz, and the PIADC animal care staff for excellent … WebJun 24, 2024 · CSFV structural protein E2 was observed colocalizing with CALCOCO2/NDP52 in PK-15 cells. CALCOCO2/NDP52 silencing also decreased the autophagic markers CD63, LC3 and BECN1; however, colocalization between LC3 and ubiquitin was also decreased. ... Skeletal and kidney-enriched inositol phosphatase. …

WebCSFV E2, which is not identical to the one in BVDV, was shown to interact with a host cell receptor (15), presumably CD46. (Overall, the amino acid sequences of BVDV and CSFV E2 are 65% identical.) The most similar structure to domain II is the ﬁrst Ig domain of receptor for advanced glycation end products

Webwe identiﬁed protein phosphatase 1 catalytic subunit beta (PPP1CB), which is part of the Protein Phosphatase 1 (PP1) complex, as a speciﬁc binding host partner for E2. We … shuttle mco to port canaveralWebApr 11, 2024 · Mono-lactate glyceride (LG) is a short-chain fatty acid ester. It has been shown that short-chain fatty acid esters play an important role in maintaining intestinal structure and function. The aim of this study is to investigate the effects of mono-lactate glyceride on growth performance and intestinal morphology and function in weaned … shuttle mdw to ordWebApr 9, 2024 · E2 is the major structural glycoprotein of the classical swine fever virus (CSFV). E2 has been shown to be involved in important virus functions such as replication and virulence in swine. Using the yeast two-hybrid system, we previously identified several host proteins specifically interacting with CSFV E2. Here, we analyze the protein … shuttle mco to ocalaWebSep 1, 2024 · Objective To develop a simple method for efficient expression of classical swine fever virus (CSFV) E2 protein. Results The pFastBac HT B vector (pFastHTB-M1) … theparisonline.comWebFeb 5, 2024 · The envelope protein E2 appeared as valuable antigen for targeting BVDV and CSFV. For both viruses, E2 was described to be the key immunogen involved in neutralization upon infection [52,53,54,55].To vary the complexity of the constructed fusion proteins, N-terminal parts of the respective E2 of different lengths were chosen to be … shuttle meaning in bengaliWebOct 10, 2024 · The cells were incubated CSFV E2 antibody (JBT, 9011) at 4°C overnight (in dilute E2 protein antibody with PBS at a ratio of 1:200 (40 μL/well). ... (Beyotime, P0013) buffer with protease and phosphatase inhibitor cocktail (Beyotime, P1050). The protein concentration was determined with Pierce™ BCA Protein Assay Kit (Thermo Fisher, … shuttle mco to port canaveral hotelsWebSep 1, 2005 · APCs transfected with either of the mRNA molecules restimulated CSFV-specific T cells to produce gamma interferon and specific cytotoxic activity against CSFV-infected target cells. The presence of CTL epitopes on E2 was confirmed by using d/d-haplotype MAX cells expressing E2 constitutively as target cells in d/d-haplotype CTL … shuttle mco to vero beach