P2a-tdtomato
WebJan 28, 2024 · To generate the PDGFR α -P2A-tdTomato-P2A-Thy1.2 hESC reporter (PD-TT), the ESC line WA09 (H9) was engineered to integrate the IAP tag at the 3′-end of the PDGFRα gene using CRISPR-based genome... WebFor easier culture, we generated an OCT3/4-P2A tdTomato IRES Zeocin R Cyn ES cell line using CRISPR/Cas9 genome editing technology. The stop codon of the endogenous OCT3/4 locus was replaced with the P2A tdTomato IRES Zeocin R pA cassette by homologous recombination. This cell line enables us to isolate pluripotent stem cells and …
P2a-tdtomato
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WebJan 1, 2024 · The two-photon laser was tuned to the excitation wavelength for tdTomato (1000 nm). All experiments were performed using a 1.1-numerical aperture (NA) 25× objective lens immersed in ACSF to obtain 62.5X high-magnification (166.4 μm × 166.4 μm; 512 × 512 pixels; 1.5-μm step) images for neuronal analysis. WebIn the PDGFRb-P2A-CreER T2 -tdTomato mouse, an induction efficiency between 78.9 and 94.1% was achieved. TdTomato expression in the retina was restricted to PCs and vascular smooth muscle cells in the NG2-CreER™-tdTomato mouse, however, in the PDGFRb-P2A-CreER T2 -tdTomato mouse, tdTomato was also expressed in Müller glial cells. …
WebJun 19, 2014 · The use of 2A peptides in Drosophila has been described in four recent reports. Gonzalez et al., 2011 ( [22]) utilized a 2A peptide-containing vector to co-express … WebDec 18, 2024 · In zebrafish, mutation of tryptophan hydroxylase 2 ( tph2 ), which is required for serotonin (5-HT) synthesis in the RN, results in reduced sleep, sleep depth, and homeostatic response to sleep deprivation ( Oikonomou et al., 2024 ). Pharmacological inhibition of 5-HT synthesis or ablation of the RN also results in reduced sleep.
WebThe Prkcg-P2A-tdTomato mice the authors constructed provide a useful tool for further analysis on how the spinal allodynia gate works. The current study indicated that nerve injury enhanced the excitability of spinal protein kinase C γ expressing interneurons due to disinhibition of the feed-forward … WebJun 27, 2016 · Here we report the production of a BRAF-P2A-tdTomato (Braf TOM) mouse model. We show that the Braf TOM mouse expresses bright tdTomato in several organs …
WebA iCre-P2A-tdTomato-polyA expression cassette was knocked into the Ly6g gene start codon site. Research Application ：Cre tool mice Validation Data Figure 1. FACS …
WebTamoxifen诱导Cre-ERT2小鼠 使用指南. Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。 hcs 7 user guideWebNov 29, 2024 · Here, we have demonstrated that transient selection with puromycin or zeocin can facilitate highly efficient scarless KI of relatively large reporter genes (over 2 kb in the case of BRN3B-P2A-tdTomato-P2A-Thy1.2) into both human and mouse PSCs. These KI cell lines differentiated normally, and did not show karyotypic abnormalities or display … golden age arms catalogWebApr 1, 2024 · OHCs were tdTomato-positive, and they were identified and picked under a fluorescence microscope. For picking IHCs, we used vGlut3-P2A-iCreER/+, Rosa26-loxp-stop-loxp-tdtomato/+ mice (15, 17). The ... golden age arms coWebThe Prkcg-P2A-tdTomato mice the authors constructed provide a useful tool for further analysis on how the spinal allodynia gate works. The current study indicated that nerve … hcs8.0.2Web,南模生物是国内模式生物领域领先的生物科技公司，专业从事遗传修饰动物模型(转基因小鼠、斑马鱼、线虫)的研发、饲养 ... hcs 7 user manualgolden age apartments white bluff tnWebCre-p2a-tdTomato (Plasmid #176837) Print Enlarge View all sequences Purpose Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato … hcs8