Phospho-kinase antibody array
WebKinase Antibody Array - Full Moon BioSystems Kinase Antibody Array Kinase Antibody Array features 276 well-characterized kinase and related antibodies associated with … WebMay 11, 2024 · An antibody specific to the phosphorylation-of-interest is added to the membrane. The phosphorylation antibody is then bound by a secondary antibody conjugated to horseradish peroxidase (HRP). Antibody binding is detected when an HRP substrate is added, resulting in chemiluminescence.
Phospho-kinase antibody array
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WebR&D Systems' Mouse Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY014) is a screening tool designed to simultaneously detect the relative phosphorylation of 39 different RTKs. The Kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. WebAntibody Arrays; IHC/Pathology Ab. IHC Ab; Pathology Ab; Circulating Rare Cell Ab. ... (phospho-Tyr393/429) Antibody detects endogenous levels of ABL1/2 only when phosphorylated at tyrosine393/429. Form: ... The protein is highly similar to the ABL1 protein, including the tyrosine kinase, SH2 and SH3 domains, and has a role in cytoskeletal ...
WebNational Center for Biotechnology Information WebApr 11, 2024 · Human Phospho-Kinase Array - Eight nitrocellulose membranes; Part A contains 21 antibodies printed in duplicate, and Part B contains 18 antibodies printed in …
WebHuman Phospho-Kinase Antibody Array (Membrane, 43 Targets) (Y0225) (AbAr-0225-YC) Antibody array is a specific form of protein microarray. In this technology, capture … WebOct 16, 2024 · The Proteome Profiler Mouse Phospho-RTK Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose …
WebProduct name Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) Sample type Tissue Extracts, Cell Lysate Assay type Semi-quantitative Species reactivity Reacts with: Human Product overview ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors.
WebJun 18, 2024 · Here, we conducted integrated analysis of human phospho-kinase antibody array, transcriptome analysis with RNA-seq, and proteomic analysis of protein pulldown to study the molecular detail of PSPC1-potentiated phenotypical transformation, adhesion, and motility in human hepatocellular carcinoma (HCC) cells. We found that PSPC1 … how many times is vodka distilledWebJun 17, 2016 · Proteome profiling was performed using Human Phospho-Kinase Antibody Array Kit (R&D Systems) according to manufacturer’s instructions. Briefly, cells were grown in 10-cm culture dishes, rinsed ... how many times is vodka typically distilledWebThe store will not work correctly in the case when cookies are disabled. how many times is yah in the bibleWebJul 9, 2024 · Methods Using a phospho-kinase antibody array we identified changes in signalling associated with HIP1 overexpression PNT1 cells. For validation Western blots were used together with knockdown or inhibitor treatments and phenotypic assays for cellular transformation. The cell line was xenografted to assess tumour growth. how many times jee mains is conductedWebCapture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1 mL of 1 sample to each … how many times is yahweh in the bibleWebGet $500 voucher for order over USD $5,000 in a year. AKT/PKB Kinase (Human) Assay/Inhibitor Screening Kit is a single-site, non-quantitative immunoassay for AKT activity. Plates are pre-coated with "AKTide-2T" (10), which can be efficiently phosphorylated by AKT1, 2 and 3 on a microtiter plate. The detector antibody is AT-3E2, an antibody that ... how many times jennifer lopez had diarrheaWebFeb 28, 2024 · To validate the kinase array phospho-Y2H results, AAR2 and PRPF8 were tested in a co-expression and immunoprecipitation assay in HEK293 cells. Co-expression of either SGK2 or CK2α1 reduced the amount of wild-type AAR2 co-immunoprecipitated with PRPF8 in comparison to the nonphosphorylatable mutant S284A (Fig 5F). Together, these … how many times itr can be revised